Peptide bioregulators: grounding of the efficiency and recommendations for use in animals

Why are peptide bioregulators effective in animal application? Peptide bioregulators were initially developed as molecules capable of restoring the functions of organs and tissues in humans during the process of aging, in the event of diseases and for prevention of pathology. Polypeptide complexes were isolated from animal organs (cattle), exhibiting maximum effect on the organ from which they were isolated. Consequently, the effect of peptide therapy in animals will be analogous to that in humans, and possibly even stronger.

The initial focus of research on peptide bioregulators centered on experimental animal models (mice, rats, pigs, rabbits, minks, monkeys, birds, etc.). Subsequent to this, the research expanded into the field of human pathology. The accumulated body of knowledge from over four decades of research on peptide bioregulators has encompassed a substantial volume of material concerning their efficacy in the context of aging and diseases in animals. The physiological activity of polypeptides and short peptides has been thoroughly examined in 17 species of organisms, including bacteria, insects, amphibians, rodents, primates, and other humans, as shown in the scheme below.

The scheme of the universal mechanism of peptide bioregulation for animals, insects, plants and humans [Khavinson V.Kh., Popovich I.G., Linkova N.S. et al. Peptide Regulation of Gene Expression: A Systematic Review. Molecules. 2021. 26(11). 7053: 1-22; https://khavinson.info/assets/files/skan/2021-khavinson_popovich.pdf].

The primary pathological conditions in animals for which the use of peptide bioregulators is applicable. Our European colleagues specializing in veterinary medicine have identified 11 major requests for which peptide bioregulators may be effective.

1. Fear of separation (separation anxiety) in an animal. This condition has the potential to result in the onset of endocrine, immune disorders or gastrointestinal abnormalities in a pet. This becomes a challenging problem for the pet owner, as it forces him or her to give up going on vacations and business trips. In order to assist your pet in coping with separation anxiety, administration of nervous system peptides is recommended. Specifically, Pinealon should be given for a period of two weeks, in the morning and evening, and Cerluten for a further two weeks, in the same manner.
2. Emotional control (calming) of animals. Emotional instability in canines and felines can result in the animal causing damage to the interior of the home, e.g. scratching furniture. This situation is likely to cause considerable distress to the owner, while his or her frustration is likely to exacerbate the animal’s psychological well-being. CBD oils containing cannabinoids have been trialled as a potential solution to this issue. However, this approach hasn’t received a sufficient evidence-based scientific basis for its effectiveness. The recommended course of action to address this problem is combining the use of Revilab SL02 or Revilab ML03 and Cerluten in the morning and evening until the symptoms subside or for a period of one to three months.
3. Enhancing the gloss of coat and maintaining overall skin condition in cats, dogs, horses and other animals. In order to maintain the quality of the animal’s coat, it is important to ensure that the animal is receiving adequate nutrition and a sufficient amount of vitamins. In addition, it is essential to address any imbalances in the intestinal microflora to eradicate any parasites. For improvement of GI and metabolic function the administration of Revilab SL05 or Revilab ML06 in the morning, followed by Chonluten, Suprefort and Svetinorm in the evening, is recommended until the manifestation of symptoms subsides or for a period of one to three months. The incorporation of Cartalax cartilage and bone peptide and Vesugen vascular peptide in the morning for one to three months can as well be recommended to enhance the quality of the animal’s coat.
4. In the event of skin injuries and abrasions, which frequently occur in animals during the hunting process, the application of peptides designed to expedite wound healing and mitigate scarring severity in hunting animals can be beneficial. We recommend taking Revilab ML09 or Cartalax and Vesugen in the morning and Revilab SL03 or Crystagen and Endoluten in the evening until the manifestation of symptoms is resolved or for a period of one to three months.
5. In order to prevent ocular diseases in animals, the administration of Pinalex ophthalmic drops is recommended with one drop instilled in each eye in the morning (in the absence of infectious ocular diseases).
6. The administration of Revilab ML09 or Cartalax and Vesugen in the morning, followed by Revilab SL03 or Crystagen and Endoluten in the evening is recommended for a period of one to three months as a preventative measure against dental disease in animals.
7. In order to eliminate bad breath, it is recommended to correct nutrition, restore intestinal microflora and get rid of parasites. In addition, the use of Revilab SL05 or Revilab ML06 in the morning along with Chonluten, Suprefort and Svetinorm in the evening is advisable until the symptoms disappear or for a period of one to three months.
8. Animal viral diseases. Immune-protective peptides are an effective means for strengthening the immune system and combating viral infections in animals. To address this issue, the application of polypeptide complexes — specifically, Vladonix thymus polypeptide and Crystagen tripeptide — is hereby recommended, administered in the morning for a period from one to three months. It has been previously established that the EW peptide incorporated into Vladonix has the capacity to regulate the morphology of thymus, spleen, and lymph node cells in mink models of Aleutian disease. Subsequent to the peptide administration on day 5, a 38.8% augmentation in the concentration of immunoglobulin A (IgA) was observed in the blood of mink, and by day 10, this increase reached 72.8%, in comparison with the baseline concentration prior to peptide application. The protective role of antibodies of the IgA class is well-documented; these antibodies protect mucous membranes from viruses and bacterial toxins, stimulate phagocytosis, and provide local resistance to infection [Lapina T.I., Ponomarenko D.G. Influence of thymogen and polyoxidonium on some indicators of humoral immunity of mink at Aleutian mink disease. Natural science and humanism. Collection of scientific works. Tomsk, 2006. Vol. 3. No. 2. P. 49-50].
9. Increasing speed and performance in horses. To maintain muscle function and endurance, improve speed in racehorses the use of peptide bioregulators Gotratix, Cartalax, Vesugen, Pinealon and Vladonix is recommended in the morning and evening from one to three months.
10. Peptides for cancer prevention in animals. Polypeptide complexes of epiphysis (Endoluten) and thymus (Vladonix) inhibit the growth of both spontaneous and induced tumors in animals. Administration of these polypeptide complexes reduced the incidence of mammary adenocarcinomas in mice by 1.8 to 3.8 times. The application of the epiphysis polypeptide complex in rats led to a 10% decrease in the total number of tumors following radiological or chemical carcinogenesis. This peptide bioregulator has been shown to inhibit the growth of squamous cervical cancer and microalveolar breast cancer in animals in cases of transplantable tumors. The polypeptide complexes Endoluten, Vladonix, Crystagen, Chonluten, and Revilab SL03 or Revilab ML01, ML02 are recommended for tumor prevention in animals for one to three months, two to three times per year.

Dosage calculation of peptide bioregulators depending on animal body weight. The dosage of lingual peptide bioregulators is selected based on the weight of the animal. If necessary, the dosage and frequency of administration can be increased. In preclinical studies, peptide bioregulators have been shown to be non-toxic. In acute toxicity tests (single administration of the peptide) in animals, a dose 5000 times higher than the therapeutic dose was safe. In subacute (peptide administration for 3 months) and chronic (peptide administration for 6 months) toxicity studies, exceeding the therapeutic dose by 300-3000 times did not cause any side effects.

All peptide bioregulators can be added to the animal’s soft food. It is important to make sure that the animal has completely eaten the food and received the required dosage of peptide. The course of administration varies from 1 to 6 months, depending on the severity of the condition. To maintain the achieved effect and prevent recurrence of the disease, it is recommended to repeat the administration after 1-3 months.

Note. Peptide bioregulators in capsule form can also be administered to animals. Each capsule contains 0.1 milligrams of the active peptide substance, equivalent to 100 micrograms. The dosage of peptide bioregulators in capsules is determined by the animal’s weight:

The material was prepared by:

• A senior expert consultant at the Peptides company, professor, Doctor of Biological Sciences Natalia Linkova
• A genetic counselor, medical adviser of the Peptides company, Candidate of Medical Sciences Irina Saburova
• The president of the Peptides company, oncologist, Candidate of Medical Sciences Roman Pinaev